Protein folding in confined and crowded environments
نویسندگان
چکیده
منابع مشابه
Protein folding and binding in confined spaces and in crowded solutions.
Simple theoretical models are presented to illustrate the effects of spatial confinement and macromolecular crowding on the equilibria and rates of protein folding and binding. Confinement is expected to significantly stabilize the folded state, but for crowding only a marginal effect on protein stability is expected. In confinement the unfolded chain is restricted to a cage but in crowding the...
متن کاملNanoparticle diffusion in crowded and confined media.
We identify distinct mechanisms controlling slowing of nanoparticle diffusion through complex media featuring both rigid geometrical confinement and soft mobile crowders. Towards this end, we use confocal microscopy and single particle tracking to probe the diffusion of 400 nm nanoparticles suspended in Newtonian water, in a Newtonian glycerol/water mixture, or in a non-Newtonian polymer soluti...
متن کاملRobust protein protein interactions in crowded cellular environments.
The capacity of proteins to interact specifically with one another underlies our conceptual understanding of how living systems function. Systems-level study of specificity in protein-protein interactions is complicated by the fact that the cellular environment is crowded and heterogeneous; interaction pairs may exist at low relative concentrations and thus be presented with many more opportuni...
متن کاملProtein-protein interactions affect alpha helix stability in crowded environments.
The dense, heterogeneous cellular environment is known to affect protein stability through interactions with other biomacromolecules. The effect of excluded volume due to these biomolecules, also known as crowding agents, on a protein of interest, or test protein, has long been known to increase the stability of a test protein. Recently, it has been recognized that attractive protein-crowder in...
متن کاملTaming Lévy flights in confined crowded geometries.
We study two-dimensional diffusive motion of a tracer particle in restricted, crowded anisotropic geometries. The underlying medium is formed from a monolayer of elongated molecules [Cieśla J. Chem. Phys. 140, 044706 (2014)] of known concentration. Within this mesh structure, a tracer molecule is allowed to perform a Cauchy random walk with uncorrelated steps. Our analysis shows that the presen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Archives of Biochemistry and Biophysics
سال: 2008
ISSN: 0003-9861
DOI: 10.1016/j.abb.2007.07.013